摘要
对菌株AspernigerLQ1 2 5产生降解酶适宜条件进行了研究。在起始 pH5 .0的 1 0 0mL/2 5 0mL培养液中 ,加入水解单宁 (橡单宁 ) 5 .0 g/L、蔗糖 2 0 g/L、蛋白胨 6g/L ,在 3 0℃、1 2 0r/min摇瓶中培养 48h ,单宁酶活力达到最大 ;外加碳源有利于菌株对单宁的利用。
Model compound was introduced in the experiment for investigating properties of Asperniger LQ125 degrading hydrolysable tannin. It is found that additional carbons were favorable to degradation of the valonia tannin. The suitable condition for producing tannin hydrolase, tannase, was also obtained as 100mL /250mL, pH 5.0 of liquid culture medium, which was mainly composed of 5 g/L polyphenol,20 g/L sucrose and 6g/L peptone,kept it oscillating for 48h at 30℃. This hydrolase showed a high stability at pH 4.0～5.0 and 30℃.
出处
《中国皮革》
CAS
北大核心
2004年第15期10-12,21,共4页
China Leather
