NO、NOS、SOD及氧自由基在分泌性中耳炎中耳积液和血清中的表达及意义

Expression of NO, NOS, SOD and oxygen free radicals in serum and middle ear effusion of secretory otitis media and its significance

目的 :探讨一氧化氮 (nitric oxide,NO)、一氧化氮合酶 (nitric oxide synthase,NOS)、超氧化物歧化酶 (superoxidedismutase,SOD)及氧自由基在分泌性中耳炎 (secretory otitis media,SOM)发病机制中的作用。方法 :对实验性 SOM和SOM患者中耳积液和血清中 NO、NOS、SOD及氧自由基进行检测 ,并比较单纯应用抗生素与 NO合成抑制剂 +抗氧化剂 +抗生素治疗 SOM后血清中各指标的变化情况。结果 :实验性 SOM和 SOM患者血清中 NO、NOS、SOD、MDA含量均明显高于正常对照组。鼓室积液中 NO、NOS、SOD、MDA含量均明显高于血清中含量。 NO合成抑制剂 +抗氧化剂 +抗生素治疗SOM后血清 NO、NOS、SOD、MDA含量明显低于单纯应用抗生素组 ,差别有统计学意义。结论 :NO、NOS、SOD及氧自由基在分泌性中耳炎发病和转归中起重要作用 ,N-硝基 - L -精氨酸甲酯 (L - NAME)、维生素 C、维生素 E及 SOD对 SOM的治疗有效。

Objective: To study the role of NO,NOS, SOD and oxygen free radicals in the pathogenesis of secretory otitis media (SOM). Methods: The levels of NO, NOS, SOD and oxygen free radicals in the serum and middle ear effusion in experimental SOM and the patients with SOM were detected by immunological analysis. Results: The serum leves of NO, NOS, SOD and MDA in experimental SOM and the patients with SOM were markedly higher than those in the control group. The levels of NO, NOS, SOD and MDA in the middle ear effusion were markedly higher than those in the serum. The serum levels of NO, NOS, SOD and MDA in the combined therapy group of NO synthesis inhibitor, antioxygen and antibiotic were significant lower than those in the simple antibiotic therapy group. Conclusion: The NO, NOS, SOD and oxygen free radicals play an important role in the pathogenesis and recovery of SOM. L-NAME, vitamine C, vitamine E and SOD are effective to SOM.