摘要
在猪绦虫融合抗原 pCC2 7(本室从六钩蚴cDNA表达文库筛选的 3个保护性抗原经拼接所得 )基因片段 5′末端引入 1个通用型辅助性T淋巴细胞表位、2个谷胱甘肽还原酶的辅助性T淋巴细胞表位 (TGG) ,构建成DNA疫苗。通过肌肉注射途径将这种DNA疫苗免疫小鼠和仔猪。动物试验结果表明 ,TGG表位可提高小鼠血清 pCC2 7抗体IgG、IgG1、IgG2a的水平 ,且长时间保持较高应答水平。用该疫苗免疫仔猪获得了 89%的保护率。
An universal helper T lymphocyte epitope and two GST e pitopes(TGG) were spliced to the 5end of pCC27 fusion gene to construct a DNA vaccine pVAX-STGG-CC27. Mice were vaccinated intramuscularly with the modified pCC27 DNA vaccine.Then the protective immunity and the humoral immune respo se in pigs were done. The animal test showed that the TGG epitopes could increas e the level of IgG and more importantly, of IgG 2a,IgG 1 pCC27-specific a ntibodies. The higher duration of IgG response induced by the modified DNA vacci ne pVAX-STGG-CC27 was longlived. The protective rate induced in immunized newb orn pigs was 89%.
出处
《药物生物技术》
CAS
CSCD
2004年第4期225-228,共4页
Pharmaceutical Biotechnology
基金
江苏省"十五"攻关课题 苏科技 [2 0 0 0 ] 3 13号
