PDGF-B链基因三链形成寡核苷酸对C_6胶质瘤细胞增殖和细胞周期的影响

The effects of triplex forming oligonucleotide (TFO) on the proliferation and cell cycle with C_6 glioma cells

目的 观察PDGF B链基因三链形成寡核苷酸 (triplex formingoligonucleotide ,TFO)对C6胶质瘤细胞增殖和细胞周期的影响。方法 应用免疫荧光流式细胞技术观察PDGF B链基因TFO对C6胶质瘤细胞PDGF B、PCNA表达的影响。应用流式细胞技术观察PDGF B链基因TFO对C6胶质瘤细胞细胞周期的影响。结果 PDGF B链基因TFO对C6胶质瘤细胞PDGF B链基因、PCNA的表达有明显抑制作用 ,而且抑制作用存在浓度依赖性。PDGF B链基因TFO能使C6胶质瘤细胞S期的百分率明显降低 ,阻止细胞由静止期 (G0 G1期 )进入 (S期 )。结论 PDGF B链基因TFO能够抑制C6胶质瘤细胞PDGF B链基因的表达 ,阻碍细胞进入S期 。

Objective To clarify effect of triplex forming oligonucleotide (TFO) on the proliferation and cell cycle with C 6 glioma cells. Methods The expressions of PDGF-B chain and PCNA in C 6 glioma cells after adding a set of dosages of TFO were quantified by immunofluorescence flow cytometric analysis.Cell cycle of C 6 glioma cells after adding a set of dosages of TFO were detected by flow cytometric analysis. Results TFO can obviously inhibit the expressions of PDGF-B chain and PCNA in C 6 glioma cells in concentration-dependent fashion. TFO can obviously inhibit the transit of cells from the G 0 -G 1 to S phase in concentration-dependent fashion. Conclusion TFO can effectively suppress the PDGF-B chain expression and the cell proliferation C 6 glioma cells. TFO also can effectively induce the C 6 glioma cells arrest in the G 1 phase of the cell cycle.