热应激和热习服对大鼠自身花环和抗体生成细胞的影响

EFFECTS OF HEAT STRESS AND ACCLIMATIZATION ON AUTOROSETTE AND ANTIBODY FORMING-CELL IN RATS

36只8周龄雄性SD大白鼠,随机分为常温对照组、热应激组、热习服组,每组12只。热习服组每天在热室中热暴露60min,连续14天;其中半数动物继续热暴露至21天,每天热暴露时间延长至100min,于热暴露第14天和第21天热应激组各6只动物与热习服组同时热暴露。热习服后,热习服组动物的肛温增值比首次热暴露降低非常显著(P<0.01和0.001),主要免疫器官重量较高,而抗体生成细胞光密度值略低于其它二组。热习服组、热应激组和对照组自身花环(%)分别为25.50±10.36、17.75±13.25和5.57±3.74,热习服组与对照组差异非常显著(P<0.01),提示:适度的热暴露可使机体自身花环形成细胞增加,热习服增加更为明显。

36 male SD rats(8 wks) were randomly divided into 3 groups (n = 12, each) i.e.control group of normal temperature (NT) , heat stress group (HS) and heat acclimatization group (HA) . HA was exposed to heat totting up to CO min daily in a hot chamber for 14 consecutive days, and then 50 percent of the rats were kere under heat exposure until 21 days, and heat exposure time daily prolonged to 100 min. On the heat exposure of the 14th and 21st days, 6 animals both in HS and in HA were exposed to heat in the same chamber. After heat acclimatization, rectal temperature increment in HA decreased significantly when compared with that of the first heat exposure (P<0.01 and P<0.001) , the weights of main immunological organs were heavier, while QHS OD value was lower than that in other 2 groups. A-R (%) were 25.50 ± 10.36, 17.75 ± 13.25 and 5.57 ± 3.74 in HA, HS and NT respectively. The difference between HA and NT had a statistical significance (P<0.01) . It is suggested that the suitable heat exposure could enhance the ARFC in organism, and that the ARFC could enhance more obviously after heat acclimatization.