用ELISA法与胶体金法检测服务业人群HBsAg的结果比较

Examining HBsAg with the Methods of ELISA and GICA and Comparing the Results

目的 :探讨在服务业人群中检测乙肝HBsAg的方法 ,对ELISA法与胶体金法两种主要方法进行比较分析。方法 :对迎泽区 2 0 0 3年 1 65 0 0名服务业人员同时采用ELISA法和胶体金法检测HBsAg。结果 :2 0 0 3年检测服务业人员 1 65 0 0人 ,ELISA法阳性 2 1 5人 ,阳性率 1 .3%;胶体金法阳性 2 1 2人 ,阳性率 1 .2 %。符合率 98.5 %,二者阳性结果无显著性差异 ( χ2 =0 .0 2P >0 .5 )。结论 :ELISA法是定量检测 ,灵敏度和特异性较高 ,但反应时间较长 ,需要较好的仪器及与仪器配套的试剂盒 ;胶体金法目前只能定性检侧 ,但操作简便、观察直观、快速、省时 ,不需特殊设备 ,其特异性、灵敏度均已达到ELISA水平 ,作为筛查健康人群HBsAg的重要方法 。

Objective:The methods of ELISA and GICA are two main methods in examining HBsAg,comparing these two kinds of methods.Method:HBsAg was examined in 16500 employees of yingze area in 2003 with ELISA and GICA at the same time.Result:of 16 500 emplyees,HBsAg was positive in 215 people(1.3%)with ELISA method and 212 people(1.2%)with GICA method(P>0.5).Match rate was 98.5%.Conclusion:The method of ELISA is of quantitative analysis,the sensitivity and the specificity were high,but the reaction time is longer and good instrument was in demand. The method of GICA is of qualitative analysis,but operation is simple and saving time without special equipments.Its specificity and sensitivety were identical with ELISA.To examine HBsAg in health people,The method of GICA has good prospect