下调脑区的激活及其中药作用机制的研究

The Mechanism of Traditional Chinese Drug in Activating the Cerebral Area of Functional Down-Regulation

目的 :从下调脑区的激活 ,研究复健片治疗缺血性中风后遗症的作用机制。方法 :电凝法制备MCAO大鼠模型。 180只大鼠随机分为模型组、药物组和假手术组。于造模成功后 5周药物组按 4.5g生药 /kg灌胃给予复健片 ,余两组分别灌胃给予同等量生理盐水 ,日 1次 ,共 2周。以BWT评分法进行神经行为学评价 ,结合局部脑血流量 (rCBF)测定 ,免疫组化和原位杂交法分别观察大鼠脑组织生长相关蛋白 (GAP -4 3 )、突触素P38(SYN)的表达和大鼠脑微血管内皮细胞ET -1、ecNOS、P -gpmRNA的变化。结果 :模型组大鼠病灶边缘区及远隔区存在低灌注现象 ,突触活性降低 ,脑微血管内皮细胞对脑微循环的调节机能下降 ;药物组可明显改善这种低活动状态 ,并能显著提高大鼠运动功能。结论 :复健片对缺血性中风后遗症模型神经功能有显著改善作用 ,激活下调脑区是其重要机制。

Objective:To study the action mechanism of fukang tablets curing sequela of ischemic cerebrovascular disease from the activation cerebral area of functional down-regulation.Methods:180 rats were divided into model group,drug group and pseudo-operation group at random.The model of rats of MCAO were successfully made by electrocoagulation.After five weeks,the rats of drug group had been taken orally fukang tablets by 4.5g/kg crude drug one time daily for two weeks,the rest groups had been taken orally equivalence saline one time daily for two weeks.Neuroethology were evaluated with BWT scoring standard,and regional cerebral blood flow (rCBF)were measured,the expresses of GAP-43,SYN in the brain tissues of experimental rats and the change of ET-1?ecNOS?P-gp mRNA in the brain microvessel endothelial cell of experimental rats were observed by means of immunohistochemical method and hybridization in situ.Results:The focus remoteness areas of the model rats presented hypoperfusion phenomena.The synapse activity and the function of brain microvessel endothelial cell accommodating brain microcirculation descended in the model group.However,the low activity states were obviously improved in the drug group,and the traditional Chinese drug could notably enhance the motor function of experimental rats.Conclusions:The fukang tablets can obviously improve the nerve function of the model rat of sequela of ischemic cerebrovascular disease.The activation of cerebral area of functional down-regulation is important mechanism of fukang tablets action.