桂皮酸的药代动力学和生物利用度研究

Pharmacokinetics and Bioavailability of Cinnamic Acid in Mice

目的 :研究桂皮酸在小鼠体内的药代动力学和生物利用度。方法 :RP HPLC法测定小鼠ig和iv桂皮酸后的血药浓度。色谱条件为 :HypersilC18(5 μm ,4 6mm× 2 5 0mm)柱 ,流动相 :甲醇 乙腈 水 乙酸 (2 5∶2 6∶4 9∶0 3) ,流速 1 0ml/min ,检测波长 2 70nm。结果 :桂皮酸的线性范围 2 95～ 2 95 2 5 μg/ml(r=0 9992 ) ,最低检测浓度为 0 5 μg/ml。在小鼠体内的药动学过程符合二室模型 ,绝对生物利用度 (F)为 95 98%。 结论 :本文采用的HPLC法可以测定桂皮酸在小鼠体内的血药浓度 ,获得了桂皮酸的药代动力学参数和生物利用度数据。

AIM:To study the pharmacokinetics and bioavailability of cinnamic acid in mice.METHOD:The concentration of cinnamic acid was determined by RP-HPLC. The main pharmacokinetic parameters were obtained using 3P97 program. An RP-C 18 was used as the stationary phase. The mobile phase consisted of methanol-acetonitrile-water-acetic acid(25∶26∶49∶0.3). The flow rate was 1.0 ml/min.The UV detector was set at 270 nm.RESULT:A good linearity was obtained from 2.95～295 μg/ml of cinnamic acid in mice plasma with r=0.9992. The determination concentration was 0.5 μg/ml. The plasma concentration-time curve of cinnamic acid conformed to two-compartment open model. The bioavailability(F) was 95.98%.CONCLUSION:It is the first time to determine the concentration of cinnamic acid in the plasma of mice and to study its pharmacokinetics and bioavailability.