气相色谱法测定菝葜中薯蓣皂苷元含量

Determination of Diosgenin in Smilax china by GC

目的 建立菝葜中薯蓣皂苷元的含量测定方法。方法 程序升温气相色谱法 ,CPSil 5CB熔融石英毛细管柱 ,FID检测器。结果 薯蓣皂苷元在 0 2 0～ 5 0 μg范围内具有良好的线性关系 ,r=0 9998,测定方法平均回收率为 97 2 0 %。 结论 本方法有较高的灵敏度和回收率 ,可用于菝葜中薯蓣皂苷元的含量测定。

Object To establish a method for determination the content of diosgenin in Smilax china.Method A programmed temperature gas chromatography with CP Sil 5CB capillary column was used, applying of FID detector.Result The calibration curve was linear in the range of 0.20-5.0 μg with r=0.9998 and the recovery was 97.20%.Conclusion The mothod is accuracy, sensitive and simple, it can be used for determination of diosgenin in Smilax china.