紫杉醇对人腺样囊性癌细胞株ACC-2体外辐射增敏作用

Radiosensitizing Enhancement Effect of Paclitaxel on Human Adenoid Cystic Carcinoma ACC-2 Line Cell in vitro

目的 评价紫杉醇对人腺样囊性癌细胞株 ACC- 2的辐射增敏作用。 方法 用克隆形成法检测不同时间 -浓度条件紫杉醇和 /或放射作用后的人腺样囊性癌细胞株 ACC- 2细胞存活分数 ,计算辐射增敏率 (SER)。 结果 紫杉醇对 ACC- 2细胞具有体外增殖抑制作用。人腺样囊性癌细胞株 ACC- 2的平均效应剂量 (D0 ) =1 .4 3Gy,Dq=3.6 Gy,1 0 ,1 0 0 ,1 0 0 0 nmol紫杉醇作用 2 4 h后 SER分别为 1 .2 9,1 .6 6和 1 .2 3。 结论 人腺样囊性癌细胞株 ACC- 2具有一定的辐射抗性。紫杉醇对其体外增殖抑制作用表现为时间 -浓度依赖性效应。一定条件下紫杉醇对人腺样囊性癌细胞株 ACC-

Objective To investigate the radiosensitizing enhancement effect of paclitaxel on human adenoid cystic carcinoma ACC 2 line cell. Methods ACC 2 was treated by paclitaxel and/or radiation at different time and with different concentration. The survival percent was observed and calculated by cell coloning formation, and then sensitizer enhancement ratios(SER) were evaluated. Results Paclitaxel inhibited ACC 2 proliferation in vitro. Pretreatment of cells with 10, 100, 1000 nmol paclitaxel for 24 h, the SER were 1 29, 1 66, 1 23 respectively. Conclusion Paclitaxel has radiosensitizing enhancement effect on human adenoid cystic carcinoma ACC 2 line cell in vitro.