摘要
目的 建立大鼠中枢神经系统双向电泳技术平台,从分子水平探索中枢神经系统损伤、修复的机制。方法 以固相 pH梯度等电聚焦为第一向和 SDS 均一胶 T=12.5%、10%的水平电泳为第二向成功地得到了神经组织双向电泳图谱,并借助图像分析软件研究了双向电泳重复性,另外对胶的银染和脱色作了进一步改进。结果 获得了重复性较好的检测蛋白点的肽质指纹图。结论 本研究为大鼠中枢神经损伤、修复蛋白质组研究工具平台的建立奠定了基础。
Objective To establish 2-DE technique for analyzing the proteome of rat central nerve tissue, including brain and spinal cord. Methods Two differentmethods, i.e., Precipitation with tricholoroacetic acid/acetone and ultra centrifugation were employed to extract protein from rat brain and spinal cord for 22 DE. Other factors such as method and volume of loading sample, choice of IPG gels, concentration of SDS gels, preset of electric parameters, protocol for staining and drying the gels were also improved. Results Using the proper method described above, a satisfactory 2-DE map of rat brain was obtained. Conclusion The study lays a foundation to establish a platform for central nerve system proteome research. 〔
出处
《深圳中西医结合杂志》
2004年第4期202-205,共4页
Shenzhen Journal of Integrated Traditional Chinese and Western Medicine
基金
深圳市科技局资助课题(200304087)
关键词
蛋白质组
双向电泳
中枢神经组织
Proteome
Two dimensional electrophoresis
Central nerve system
