儿童急性肾小球肾炎血管紧张素转换酶基因多态性的研究

Investigation of the association between acute glomerulonephritis in childhood and angiotensin-converting enzyme genepolymorphism

目的 探讨儿童急性肾小球肾炎与血管紧张素转换酶 (ACE)基因多态性的关系。方法 用PCR方法检测了 2 0 0 0～ 2 0 0 3年长春市儿童医院 6 6例儿童急性肾小球肾炎患儿ACE基因多态性 ,探讨ACE基因多态性与尿蛋白量、血尿、高血压、氮质血症的关系。并以 5 0名健康儿童ACE基因型作对照。结果 急性肾小球肾炎患儿ACE DD基因频率为 0 12 ,ACE II基因频率为 0 5 6 ,ACE DI基因频率为 0 32 ,急性肾小球肾炎患儿ACE基因多态性与正常对照组存在明显差异 ;ACE II基因型与肾小球肾炎的遗传易感性相关联 ,OR =2 4 77;急性肾小球肾炎ACE基因型与血尿、高血压、氮质血征有明显相关性 ,出现血尿、高血压、氮质血症的急性肾小球肾炎患者ACE基因型多为II型 ,P <0 0 1。结论 ACE II基因可能是发生急性肾小球肾炎的危险因子。检测儿童ACE基因 。

Objective To investigate the relationship between acute glomerulonephritis in children and angiotensin converting enzyme gene polymerphism(ACE).Methods We detected ACE gene polymorphism in 66 children who suffe red acute glomerulonephrites using polymerase chain reaction and discussed the association between ACE gene polymorphism and urine protein excretion weight,hematuria,hypertension,azotemia with contrast to ACE genotypes of 50 healthy children.Results The gene frequency of ACE DD in children acute glomerulonephritis was 0 12,that of ACE II was 0 56,that of ACE DI was 0 32.There was significant difference in ACE gene polymorphism in children with acute glomerulonephrites compared with control subjects.The ACE II genotype was associated with the inheritance susceptibility of acute glomerulonephritis,OR=2 477.The occurence of hematuria,hypertension and azotemia in children with acute glomerulonephrites was significantly associated with ACE genotype.The ACE genotypes of glomerulonephrites patients who manifested hematuria,hypertension,azotemia were mostly ACE Ⅱ(P<0 01).Conclusion ACE II genotype is probably the more dangerous factor that leads to acute glomerulonephritis.Detecting ACE genotype in children has important significance for detecting,preventing and curing acute glomerulonephritis in children at earlier period.