树突状细胞对脐血来源细胞因子诱导杀伤、自然杀伤细胞杀伤活性及CD45RO表达的影响

Effects of dendritic cell stimulation on cytotoxic activity of cord blood derived cytokine-induced killer cells,natural killer cells and CD45RO expression in CIK cells

目的 :探索同一来源脐血树突状细胞 (DCs)对细胞因子诱导杀伤 (CIK)、自然杀伤 (NK)细胞杀伤活性和CIK细胞上CD4 5RO表达的影响。方法 :通过细胞因子组合诱导、扩增脐血来源的DCs、CIK和NK细胞 ,杀伤效应细胞分为两组 :A组为接受DCs共孵育 6d刺激的CIK、NK细胞 ,B组为未接受任何刺激的CIK、NK细胞 ,了解DCs对相应效靶比下CIK、NK细胞杀伤K5 6 2、HL - 6 0细胞株细胞毒活性的影响 ;通过流式细胞术检测两组CIK细胞上CD4 5RO、CD4 5RA的表达率。结果 :随着效靶比的升高 ,脐血CIK、NK细胞对肿瘤细胞株的杀伤力增加。在 2 0∶1、10∶1效靶比下 ,A组CIK、NK细胞对HL - 6 0的杀伤率分别为 76 77%± 5 76 %、5 5 87%± 2 0 9% ,B组为 6 1 14 %±3 72 %、4 9 96 %± 1 5 1% ,A组对HL - 6 0的杀伤明显高于B组 ;在 2 0∶1效靶比下 ,A组对K5 6 2的杀伤明显高于B组 ;而在 10∶1效靶比下两组之间的杀伤活性未见显著差异 ;A组CIK细胞上CD4 5RO表达率显著高于B组。结论 :DCs与CIK、NK细胞共孵育可提高CIK、NK细胞的细胞毒活性及增加CIK细胞上CD4

AIM: To investigate the efficacy of dendritic cells (DCs) that augments the cytotoxic activity of cytokine-induced killer (CIK) cells, natural killer (NK) cells from a same donor and the CD45RO expression on CIK cells. METHODS: The expanded killer cells were divided into two groups: group A was pre-cocultured with DCs for 6 days, group B was the control that without any stimulation. Cytotoxicity of CIK and NK cells was measured at different effect-target ratio against K562 and HL-60. CD45RO and CD45RA expression on CIK cells in different groups were detected by flow cytometry. RESULTS: Cytotoxicity of CB derived killer cells was positive correlation with effect-target ratio. The cytotoxicity of group A against HL-60 was higher than that of group B significantly. At 20∶1 effector-target ratio, the lytic activity of group A CIK, NK cells against K562 was higher than that of group B significantly, but no significant difference between them at 10∶1 effector-target ratio. The CD45RO expression on CIK cells in groups A was significantly higher than that in groups B. CONCLUSION: CIK and NK cells cocultured with DCs can augment the killer's cytotoxicity against tumor cells and promote the CD45RO expression on CIK cells.