过氧化物酶体增殖体活化受体γ、肝脏X受体α、视黄酸X受体α活化对THP-1细胞ATP结合盒转运蛋白和CD36mRNA表达的影响

Effect of activated nuclear receptors PPARγ,LXRα,RXRα on ABC1 and CD36 mRNA expression in THP-1 cell

目的 :探讨核受体过氧化物酶体增殖体活化受体γ(PPARγ)、肝脏X受体α(LXRα)、视黄酸X受体α(RXRα)活化对THP1单核细胞ATP结合盒转运蛋白 (ABC1)和CD36mRNA表达影响的差异及其意义。方法 :体外培养的THP - 1单核细胞 ,经氟波酯 (PMA)诱导 4 8h ,使形成巨噬细胞 ,再分别用核受体PPARγ、LXRα、RXRα的相应配体曲格列酮、2 2 (R) -羟化胆固醇 [2 2 (R) -HC],9-顺式 -视黄酸 (9-CRA)作用 2 4h ,收集细胞 ,提取总RNA ,以 β -actin作内参 ,用RT -PCR法检测ABC1和CD36mRNA表达的变化。结果 :核受体PPARγ、LXRα、RXRα活化剂均可致ABC1mRNA表达增高 ,但对CD36mRNA的表达存在明显差异。PPARγ活化时CD36mRNA表达增高 ,LXRα活化时CD36mRNA表达并无明显增高 ,而RXRα活化时CD36mRNA表达降低。结论 :核受体PPARγ、LXRα、RXRα活化对ABC1和CD36mRNA表达的影响存在差异 ,提示LXRα。

AIM: To study the discrepancy and it's significance of ATP binding cassette transporter(ABC1) and CD36 mRNA expression while activate nuclear receptor RRARγ,LXRα,RXRα in THP-1 cells. METHODS: Cultured THP-1 cells were induced to become macrophage by PMA at concentration of 40 μg/L for 48 hours. Troglitazone, 22(R)-hydrocholesterol and 9-cis-retinoid acid(9-CRA),activators for PPARγ,LXRα and RXRα respectively, were incubated with the macrophage for 24 hours. Total RNA of these cells were abstracted and reverse transcriptional polymerase chain reaction were performed to inspect the expression of ABC1 and CD36 mRNA in the cells. RESULTS: Expression of ABC1 mRNA increased in the presence of troglitazone, 22(R)-HC and 9-CRA. CD36 mRNA also increased while PPARγ activated. Activation of LXRα showed almost no effect on CD36 mRNA expression, and expression of CD36 mRNA was decreased by 9-CRA.CONCLUSION: The discrepancies existing in ABC1 and CD36 mRNA expression while activate nuclear receptors PPARγ,LXRα,RXRα in THP-1 cells implyed that LXRα,RXRα were better targets to deal with to improve cholesterol reverse transportation.