纤维蛋白原对人血管平滑肌细胞株增殖和移行的影响

Effect of fibrinogen, fibrin and fibrin degradation products on the proliferation and migration of human vascular smooth muscle cells

目的 :探讨纤维蛋白原 (Fg)、纤维蛋白 (Fb)及其降解产物 (FDPs)对体外人血管平滑肌细胞 (VSMC)增殖与移行的影响。方法 :采用细胞计数法和四唑盐 (MTT)比色法测定VSMC增殖 ,通过刮伤实验 (woundingmodel)和Transwell嵌套装置观察VSMC的移行。结果 :Fg本身对VSMC增殖无促进作用 ,但可促进VSMC的移行 ,且呈浓度依赖关系 ;Fb及FDPs均可明显促进VSMC增殖和移行 ,Fb的作用还呈现浓度依赖关系 ,而FDPs作用呈现先增强后减弱的趋势 ,即在一定浓度范围内作用明显 ,高于或低于此范围作用较弱。结论 :Fb及FDPs通过促VSMC的增殖和移行作用 。

AIM: To investigate the effect of fibrinogen (Fg), fibrin (Fb) and fibrin degradation products (FDPs) on the proliferation and migration of human vascular smooth muscle cells (VSMC).METHODS: The effects of Fg, Fb and FDPs on the proliferation of VSMC were observed by means of cell counting and MTT test, migration assays were performed using the wounding model and the transwell cell culture apparatus.RESULTS: Fg itself did not stimulate the proliferation of VSMC, but stimulated VSMC migration. Fb and FDPs both stimulated the proliferation and migration of VSMC, meanwhile the effect of Fb was in a dose-dependent manner.CONCLUSION: Fb, in particular FDPs, may play an important role by stimulating the proliferation and migration of VSMC in restenosis and atherogenesis.