“RecA蛋白-互补单链DNA探针”生物信号放大系统用于压电基因传感器的研究

Research of applying"RecA protein-complementary single strand DNA" biological signal amplification system to piezoelectric gene sensor

目的 利用“RecA蛋白 互补单链DNA探针”与固定的肽核酸 (bis PNA)探针相结合 ,摸索最适的液相反应条件 ,以提高压电基因传感器基因传感表面的质量负载 ,从而提高传感器的灵敏度。方法 基因传感器阵列固定上针对乙肝病毒的bis PNA探针 ,加入靶DNA与之反应 ,然后加入预先处理过的不同浓度 (0 .5～ 6mg/ml)的“RecA蛋白 互补单链DNA探针”。结果 当RecA蛋白浓度为 3mg/ml时 ,ATPγS浓度为 1 2 .5 μM ,所引起的频率变化最显著。 结论 在反应体系中加入“RecA蛋白 互补单链DNA探针” 。

Objective To improve the sensitivity of piezoelectric gene sensor by increasing its surface load on the basis of the probe of 'RecA protein complementary single strand DNA' and to investigate the optimal condition in liquid phase.Methods Bis PNA probe for detecting HBV was immobilized on the surface of gene sensor array.Different concentrations of the 'RecA protein complementary single strand DNA' probes were added after the bis PNA probes were hybridized with corresponding target DNA.Results The frequency was reduce obviously when the concentration of RecA protein was 3mg/ml and that of ATPγS was 12.5μM.Conclusion The sensitivity of piezoelectric gene sensor array is improved significantly by using the probe of 'RecA protein complementary single strand DNA'.