动脉粥样硬化斑块中细胞凋亡及其相关基因的表达

Apoptosis and expression of its related genes in human atherosclerotic plaques

目的 :研究动脉粥样硬化 (AS)病灶中细胞凋亡的发生情况及其相关基因的表达情况 ,探讨细胞凋亡与bcl 2、p5 3、C myc基因表达的内在联系。方法 :选择因AS住院手术患者 2 0例作为研究对象〔其中男 17例 ,女 3例 ,平均年龄 (6 6 .8± 9.86 )岁 ,主要取患者的股动脉〕 ,及因意外伤害手术患者的 10例〔其中男 8例 ,女 2例 ,平均年龄 (5 1.6± 11.97)岁〕作为正常对照。采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记 (TUNEL)技术检测两组动脉标本的细胞凋亡情况 ,采用免疫组化的方法检测bcl 2、p5 3及C myc基因的表达情况 ,应用透射电镜进行两组动脉标本的细胞形态学观察。结果 :AS病灶中 ,细胞凋亡的发生率明显高于正常对照血管 (P <0 .0 1) ,bcl 2蛋白的表达明显降低 ,p5 3蛋白及C myc蛋白的表达明显升高 (P <0 .0 1) ;透射电镜证实AS病灶中有大量的平滑肌细胞发生凋亡。结论 :细胞凋亡是AS病灶中细胞死亡的一种主要形式 ,bcl 2、p5 3及C myc基因的表达可能对细胞凋亡的发生起重要的调控作用。

Objective:To study apoptosis and expressions of protooncogenes bcl-2, p53 and C-myc of cells in human atherosclerotic (AS) plaques.Method:Arterial specimens were derived from 20 patients operated as arteriosclerosis and 10 victims. Cell apoptosis was in site detected with the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and transmission electron microscope. The expressions of protooncogenes bcl-2, p53 and C-myc was detected with immunohistochemistry.Result:Compared with normal arteries, more cells were found to be positively stained with TUNEL in AS plaques (P< 0.01). Lots of smooth muscle cells in AS plaques were found to be apoptosis with electron microscope. The expression of protooncogene bcl-2 was markedly decreased in AS plaques, but the expressions of p53 and c-myc was markedly increased (P< 0.01).Conclusion:Apoptosis is a main type of cell death in human AS plaques. The expressions of protooncogenes bcl-2, p53 and C-myc may play an important role in regulating the apoptosis.