喉癌基因的差异表达

Differential gene expression in squamous cell carcinoma of larynx

目的 从分子水平上探讨喉癌的发病机制。方法 用微阵列 (Microarray)技术分析了114 31个基因在喉癌组织和其临近的正常黏膜组织表达差异 ,用RT PCR技术分析所得喉癌差异表达基因中一个基因在不同类型喉癌中的表达情况。结果 喉癌组织和其临近的正常黏膜组织基因表达相差 3倍以上者为 35个 ,其中上调基因 8个 ,下调基因 2 7个 ;相差 5倍以上者 7个 ,皆为下调基因 ;上皮膜蛋白基因 1(Theepithelialmembrane 1,EMP 1)是下调基因中一个基因 ,EMP 1在不同类型配对喉癌中皆呈低表达 ,明显低于相应的正常喉黏膜组织 (P <0 0 5 )。结论 喉癌的发生涉及多个基因参与 ,微阵列技术分析喉癌差异表达基因是可靠的方法。

Objective To reveal pathogenic mechanism of laryngeal carcinoma. Methods The involved genes were identified in larynx carcinogenesis by comparing the gene expression profile of matched primary normal epithelial cells and primary laryngeal carcinoma cells from the same patients. A cDNA microarray analysis and semi-quantitative reverse transcriptase polymerase chain reaction(RT-PCR) were used to detect differential gene expression in squamous cell carcinoma of larynx. A statistical analysis was performed by SPSS version 10 and difference was considered statistically significant ifP<0.05. Results 11 431 human genes were found with significant changes in the expression of 35 genes, with 8 genes being up-regulated and 27 being down-regulated. EMP-1 was one of the down-regulated genes. The epithelial membrane 1 (EMP-1) expression in various kinds of laryngeal carcinoma is determined by RT-PCR and the EMP-1 mRNA level in all laryngeal carcinoma cells was significantly lower than that in the matched primary normal epithelial cells (P<0.05). Conclusion Larynx carcinogenesis is correlated to multiple gene expression and it is reliable to identify differential gene expression by cDNA microarray analysis.