川芎嗪舒张离体兔阴茎海绵体平滑肌的作用及其机制的初步研究

Effect of Chuanxiongzine on the Relaxation of Isolated Rabbit Corpus Cavernosum Tissues

目的 :探讨川芎嗪 (Chuanxiongzine ,Ligustrazine)对离体兔阴茎海绵体平滑肌条的舒张效应及其作用机制。 方法 :采用离体家兔阴茎海绵体肌条张力记录法 ,观察川芎嗪对去氧肾上腺素 (phenylephrine,PE)诱导收缩的阴茎海绵体肌条的舒张作用 ;应用亚硝基左旋精氨酸甲酯 (L NAME)、ODQ预处理和去除内皮 ,分别记录川芎嗪对阴茎海绵体肌的舒张作用。采用放射免疫法测定川芎嗪对阴茎海绵体平滑肌肌条中cGMP和cAMP含量的影响。 结果 :川芎嗪对阴茎海绵体平滑肌具有浓度依赖性舒张作用 ,其EC50 为 1 .5 8× 1 0 -4mol/L ,ODQ可部分抑制川芎嗪对肌条的舒张效应 (P <0 .0 5 ) ,而L NAME预处理和去除内皮对川芎嗪舒张肌条的效应没有影响 (P >0 .0 5 )。川芎嗪处理组阴茎海绵体平滑肌肌条中的cGMP和cAMP含量分别是对照组的 1 .5和 2 .3倍 ,差异有显著性 (P <0 .0 5 )。 结论 :川芎嗪对阴茎海绵体平滑肌具有浓度依赖性的舒张效应 ,其舒张作用机制与增加cGMP。

Objective: To investigate the effects of Chuanxiongzine on the relaxation of isolated rabbit corpus cavernosum tissues. Methods: Observations were made on the relaxing effect of Chuanxiongzine on phenylephrine (PE) precontractedcorpus cavernosum and cavernosal strips preincubated with L NAME, and ODQ, using endothelium removal and experimental method of smooth muscle strips. The effect of Chuanxiongzine on cAMP and cGMP levels in corpus cavernosum was measured by 125 I radioimmunoassay. Results: Chuanxiongzine had concentration dependent relaxing effect on the cavernosal muscle contraction induced by PE (EC 50 1.58 ×10 -4 mol/L). This relaxing effect was partially antagonized by ODQ and not blocked by L NAME or endothelium removal. In PE precontracted cavernosal strips, Chuanxiongzine induced relaxation accompanied with an increase in cAMP and cGMP levels. Conclusion: Chuanxiongzine was effective in relaxing cavernosal muscle precontracted by PE in vitro . And its relaxing effect may be enhanced by increasing cGMP and cAMP levels in the corpus cavernosum.