检测血清中IL-1α、IL-6、TNF-α观察丙型肝炎病情

Detection of IL-1α,IL-6 and TNF-α in Serum for the Observation of HCV

目的 检测丙型肝炎患者血清中IL lα、IL 6、TNF α的含量及其与临床的关系。观察丙型肝炎患者病情转归。方法 采用双抗体夹心酶联分析法。结果 丙型肝炎患者血清中IL 1α、IL 6、TNF α显著高于健康对照组 (P <0 .0 1) ;IL 1α与IL 6呈正相关。IL 1α、TNF α与病情轻重呈正相关 (P <0 .0 5 ) ,慢性肝炎者 1L 6含量高 (P <0 .0 1)。结论 提示IL 1α、IL 6、TNF α高低对观察病情、判断疗效具有指导作用。

Objective To detect the amount of IL-1α?IL-6?TNF-α in serum of HCV patients and its relation with the clinical manifestation,and to observe the change of the patients' condition.Methods Double antibody ELISA was applied.Results IL-lα,IL-6 and TNF-α in serum of HCV patients were much higher than those in the control group (P<0.01);IL-lα was positively related to IL-6.IL-1α and TNF-α were positively related to the severity of the disease (P<0.01).Conclusions The level of IL-1α?IL-6?TNF-α can be used as a guide in observing patients' condition and in judging the curative effect.