失血性休克大鼠心肌和肠上皮细胞ND1、ND2、mtTFA基因表达的变化

Changes of ND1, ND2 and mtTFA gene expressions in myocardial cells and intestinal epithelial cells of rats after hemorrhagic shock

目的 探讨失血性休克后线粒体呼吸链复合体Ⅰ亚单位ND1、ND2及其调控因子 mtTFA在大鼠心肌和肠上皮细胞内基因转录物水平改变规律。方法 通过采用大鼠失血性休克模型 ,提取心肌和肠上皮细胞总RNA ,行RT PCR扩增呼吸链复合体Ⅰ亚单位ND1、ND2基因及mtTFA基因并对其基因转录物水平进行了检测。结果 大鼠失血性休克后 1～ 2h ,心肌细胞ND1基因的转录水平与正常对照组比较均显著升高 ;而肠上皮细胞ND1基因的转录水平较正常对照组显著下降 ,但两种细胞ND2基因的转录水平在失血性休克后的变化趋势基本一致。两种细胞mtTFA基因的转录水平在失血性休克 1～ 2h后的转录水平则较正常对照组显著下降。结论 在缺血缺氧性损害中 。

Objective To explore the transcription pattern of ND1, ND2 and mtTFA gene in the myocardial cells and intestinal epithelial cells of rats after hemorrhagic shock. Methods Total RNA of myocardial cells and intestinal epithelial cells in rats were extracted after hemorrhagic shock. ND1, ND2, and mtTFA gene transcription levels were measured by reverse transcription and polymerase chain reaction (RT PCR). Results During the period from 1 to 2 h after hemorrhagic shock, the ND1 gene transcription levels in myocardial cells in the hemorrhagic shock groups were higher than that in the normal control group, but the levels in intestinal epithelial cells were lower than that in the normal control group. The pattern for the changes of ND2 gene transcription in myocardial cells and intestinal epithelial cells was basically similar. Conclusion There might exist certain tissue differences in the changes of ND1 gene transcripts in myocardial cells and intestinal epithelial cells of rats with hypoxic and ischemic damage.