Fas、FasL在肾癌组织中的表达及其意义

Expressions of Fas and FasL in renal cell carcinoma and its significance

目的 探讨凋亡相关基因产物Fas和FasL蛋白在肾癌发生中的作用以及与肾癌临床病理的关系。方法 采用免疫组织化学方法对 42例肾癌组织和 2 0例正常肾组织Fas和FasL蛋白的表达进行检测。结果 ①肾癌组织Fas阳性表达率显著低于正常肾组织 (P <0 .0 1) ;肾癌组织FasL阳性表达率显著高于正常肾组织 (P <0 .0 1) ;正常肾组织Fas和FasL具有相关性 (r =0 .885 ,P <0 .0 1) ,肾癌组织的Fas和FasL无相关性 (r =-0 .2 45 ,P >0 .0 5 )。②Fas蛋白表达率在各病理分型及临床分期之间比较无显著差异 (P >0 .0 5 ) ;随肾癌细胞分级的增加 ,Fas蛋白表达率逐渐降低 (P <0 .0 1) ;有淋巴结转移与无淋巴结转移组之间比较无显著差异 (P >0 .0 5 ) ;FasL表达率在各病理分型及临床分期之间比较无显著差异 (P>0 .0 5 ) ,随肾癌细胞分级的增加 ,FasL表达率逐渐增高 (P <0 .0 1) ;有淋巴结转移与无淋巴结转移组之间比较有显著差异(P <0 .0 5 )。结论 Fas和FasL蛋白相互作用失衡在肾癌的发生、发展中起重要作用 。

Objective To evaluate the role of Fas and FasL in renal cell carcinomas (RCC) and its relationship with clinicopathology. Methods Fas and FasL expressions in 42 cases of RCC and 20 cases of normal kidney tissues were measured by SP immunohistochemical technique. Results ①Fas expression rate in renal cell carcinomas was lower than that the in the normal kidney tissues ( P <0.01), but FasL expression rate in renal cell carcinoma was higher than that in normal kidney tissues ( P <0.01). The expressions of Fas and FasL had a correlation in normal kidney tissues ( r =0.885, P <0.01), but not in renal cell carcinomas ( r =-0.245, P >05). ②The protein expression rate of Fas and FasL showed no significant difference between the pathological types and clinical stages ( P >0.05). With the advance of RCC cell grade, the Fas protein expression rate increased ( P <0.01), but the Fasl protein expression rate decreased ( P <0.01). FasL protein expression was significantly higher in the group with lymph node metastasis than that in the group without lymph node metastasis ( P <0.05), but no significant difference of Fas protein expression was found between two groups ( P >0.05). Conclusion Dysfunction of the interaction between Fas and FasL may play an important role in occurrence and progression of renal cell carcinoma. The Fas and FasL protein expressions may be associated with pathological grade.