乌鲁木齐市老年人群中载脂蛋白E基因与Alzheimer病的相关分析

An association analysis of apolipoprotein E genotype and Alzheimer's disease in old population in Urumqi

目的 分析乌鲁木齐市老年人群中载脂蛋白E(ApoE)基因与Alzheimer病 (AD)的相关性。方法应用聚合酶链式反应扩增技术及限制性片段长度多态性技术对乌鲁木齐市 6 0例散发性AD患者和 90例对照者的ApoE基因进行分型 ,分别计算两组ApoE各等位基因、基因型的频率。结果 (1)AD患者组中ApoEε2、ApoEε3、ApoEε4等位基因出现的频率分别为 6 6 7%、75 83%、17 5 0 % ,而对照组分别为 13 33%、79 4 5 %、7 2 2 % ;其中 ,ApoEε4等位基因在AD组中出现的频率明显高于对照组 (P <0 0 5 ) ,ApoEε2等位基因却低于对照组 (P <0 0 5 ) ;(2 )ApoEε4等位基因在不同性别、年龄、受教育程度中的分布差异具有显著性 (P <0 0 1,P <0 0 1,P <0 0 0 1)。结论 (1)ApoEε4等位基因与中国乌鲁木齐散发性AD有明显相关性 ,其基因型以ε3/ε4为主 ;(2 )ApoEε2等位基因在AD发生时可能具有一定保护作用 ;(3)评价AD发病风险时 ,性别、年龄、受教育程度的因素不容忽视。女性、75岁以下、低教育程度。

Objective To analyse the correlation of apolipoprotein E genotype and Alzheimer's disease(AD) in old population in Urumqi.Methods The polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP) techniques were used to detect the distribution of genotype, gene frequency of ApoE alleles in 60 cases with sporadic AD and 90 normals as control.Results The frequency of ApoEε2, ApoEε3, ApoEε4 was 6.67%, 75.83% and 17.50% respectively in AD group, while in control group, it was 13.33%, 79.45% and 7.22% respectively. The frequency of ApoEε4 allele in AD group was higher than that in control group( P<0.05), while the frequency of ApoEε2 allele in AD group was lower than that in control group( P<0.05). The distribution of ApoEε4 allele was different in the patients with different gender, age or education( P<0.01).Conclusions There was an evident correlation between ApoEε4 allele and sporadic AD. The results also provide a support of the protection effect of ApoEε2 allele in the development of AD. The effect of gender, age, and education must be taken into account when we evaluate the role of ApoE genotype examination in predicting diagnosis of AD, and the carrier of ApoEε4 with younger than 75 years old, female and lower education may have a higher risk in developing AD.