短暂脑缺血再灌注后大鼠大脑皮质ATP含量的动态变化

The dynamic changes of ATP content in rat cerebral cortex after transient ischemia followed by reperfusion

目的 探讨短暂脑缺血再灌注后大鼠大脑皮质ATP含量的动态变化及其与神经功能恢复之间的关系。方法 采用线栓法建立大鼠大脑中动脉闭塞 (MCAO)模型 ,缺血 10min后于再灌注后 0h、1h、3h、6h、12h、2 4h和 72h应用毛细血管电泳法分别测定额顶叶皮质的ATP含量 ,观察其变化规律。结果 缺血 10min后额顶叶皮质ATP的含量急剧下降至对照组的 2 0 %。再灌注后ATP的含量逐渐恢复 ,于再灌注后 1h、3h、6h和 12h恢复至对照组的 70 .5 %、6 5 .7%、84 .8%和 86 .9%。再灌注后 2 4hATP含量再次下降 ,再灌注后2 4h和 72hATP含量仅为对照组的 5 0 % ,与对照组相比差异均有显著性 (P <0 0 1,P <0 0 5 )。缺血 10min再灌注后大鼠肢体功能可逐渐恢复 ,但再灌注后 2 4h起出现不愿活动和进食等表现。结论 短暂脑缺血再灌注后大鼠额顶叶皮质存在细胞能量系统功能恢复滞后的现象。同时 ,随着再灌注的进行还出现了继发性细胞能量系统功能衰竭的现象 。

Objective To investigate the dynamic changes of ATP content in rat cerebral cortex after transient ischemia followed by reperfusion and the relationship between the change of energy and the recovery of neural function.Methods The rats were subjected to 10 min of middle cerebral artery occlusion (MCAO). At the time point of 0 h, 1 h, 3 h, 6 h, 12 h, 24 h and 72 h after reperfusion, ATP contents of frontal and parietal cortex were measured by capillary zone electrophoresis.Results At the end of 10 min ischemia, ATP content fell dramatically to less than 20% of the control level. After reperfusion, ATP content recovered gradually. After 1 h, 3 h, 6 h and 12 h of reperfusion, ATP content returned to 70.5%, 65.7%, 84.8% and 86.9% of the control level ( P=0.052, 0.030, 0.332 and 0.491). From 24 h on until 72 h after reperfusion, ATP content decreased again, reaching half of the control level ( P=0.003 and P=0.023). After 10 min ischemia, limb function recovered gradually and completely at last. From 24 h on until 72 h after reperfusion, unwillingness of action and eating was found.Conclusions The recovery of cellular energy system function is delayed even though the reperfusion is in time after transient cerebral ischemia. Furthermore, secondary failure of cellular energy system function occurrs with the reperfusion proceeding. These phenomena are probably responsible for the delayed recovery of neural function after cerebral ischemia in spite of reperfusion.