立体定向毁损杏仁核对大鼠脑内单胺递质影响的实验研究

Effect of amygdala lesion on the monoamine transmitters in rat brain

目的 :探讨立体定向毁损正常大鼠杏仁核对相关脑区单胺递质含量的影响。方法 :将 75只雄性Sprague -Dawley大鼠随机分为对照组、假手术组和手术组 ,每组 2 5只。测定立体定向毁损正常大鼠杏仁核后额叶、间脑和脑干多巴胺 (DA)、五羟色胺 (5 -HT)和去甲肾上腺素 (NE)含量的变化。DA、5 -HT测定采用荧光分光光度法 ;NE测定采用放射免疫法。结果 :毁损后大鼠额叶、间脑和脑干DA显著降低于对照组 (P <0 .0 1) ;NE显著降低于对照组 (P <0 .0 5 ,P <0 .0 1,P <0 .0 1)。毁损后大鼠额叶、间脑和脑干 5 -HT均显著高于对照组 (P <0 .0 5 ,P <0 .0 5 ,P <0 .0 1)。结论 :立体定向毁损正常大鼠杏仁核后能使相关脑区单胺递质含量发生改变。

Objective:To explore the effect of amygdala lesion on the monoamine transmitters in normal rat brain.Methods: Seventy-five male Sprague-Dawley rats were randomly divided into 3 groups, control group,sham group and operation group,25 rats in each.The Dopamine,serotonin and norepinephrine were detected in the frontal lobe,interbrain and brain stem after amygdala lesion.Results:The contents of dopamine and norepinephrine in the frontal lobe,interbrain and brain stem were significantly lower than those in control groups( P <0.01).The contents of serotonin in the frontal lobe,interbrain and brain stem were significantly higher than those in control groups( P <0.05,P <0.05,P <0.01). Conclusion:The contents of monoamine transmitters were changed after amygdala lesion.