缺血预处理对肾脏缺血/再灌注时P-selectin表达的影响

Influence of ischemic preconditioning on P-selectin expression in renal ischemia/reperfusion injury model

目的 :观察缺血预处理对肾脏缺血再灌注的保护作用及对P -selectin表达的影响。方法 :建立大鼠肾脏缺血模型 ,随机分为假手术组 (S组 ) ,缺血再灌注组 (IR组 ) ,预处理组 (IPC组 ) ,于再灌注 2 4h检测大鼠血肌酐 (Scr)、尿素氮 (BUN)、丙二醛(MDA)、超氧化物歧化酶 (SOD) ,光镜下观察肾组织学改变 ,免疫组化检查肾细胞P -选择素 (P -selectin)表达。结果 :①血Scr、BUN、MDA :IPC组明显低于IR组 (P <0 .0 5 ) ;②血SOD :IPC组明显高于IR组 (P <0 .0 5 ) ;③形态学 :IR组可见大量肾小管坏死 ,超微结构不可逆性改变 ,IPC组肾小管坏死较少 ,超微结构可逆性改变 ;④P -selectin :IPC组肾脏P -selectin表达水平低于IR组。结论 :缺血预处理减轻肾脏缺血 /再灌注损害的机制可能是降低P -selectin在肾脏的表达。

Objective:To observe the protective effects of ischemic preconditioning and P-selectin expression in renal ischemia/ reperfusion injury model.Methods: Rat model of ischemia was made by left renal artery occlusion. The rats were divided into three groups randomly: sham-operated group(group S),ischemia-reperfusion group(group IR),ischemic reconditioning group(group IPC).The left renal underwent 60 minutes ischemia and 24 hours reperfusion. The levels of Scr,BUN,Superoxide Dismutase (SOD) and Malondialdehyde(MDA) in each group were examined. In addition,pathological changes were also examined by light microscope. P- selectin expressions in kidney were tested by immunohistochemistry. Results:① Scr,BUN and MDA:The values in group IPC were significantly lower than that in group IR( P <0.05). ②Blood SOD: The values in group IPC were significantly higher than those in group IR( P < 0.05 ). ③Morphology: In group IR patch necrosis of renal tubules and the ultrastructural damages were irreversible.Whereas,in group IPC scattered necrosis of renal tubules could be seen and the most of ultrastructural damages were reversible. ④ P - selectin: The expression of P - selectin in group IPC was lower than that in group IR.Conclusion:Ischemic preconditioning could protect kidney from ischemia/ reperfusion injury via scavenging oxygen free radicals,improving the SOD's activity and reducing the levels of MDA. The mechanism might be that the herbs inhibits the expression of P - selectin in the kidneys.