摘要
目的 观察IL 1β和TNF α对培养的人视网膜色素上皮 (RPE)细胞波形蛋白表达的影响。 方法 采用免疫组织化学和彩色图像分析技术检测IL 1β和 /或TNF α( 2 0ng/mL)对RPE细胞波形蛋白表达的影响。 结果 波形蛋白表达的灰度值分别为 :IL 1β +TNF α处理组 76 9± 8 1,IL 1β处理组 78 4± 8 1,TNF α处理组 90 2± 6 7。与对照组 110 2±10 7比较均有显著性差异 (P <0 0 1)。结论 IL 1β和 /或TNF α可上调波形蛋白的表达 ,在增生性玻璃体视网膜病变(PVR)中可能参与RPE细胞增殖过程中中间丝的改变。
ObjectiveTo observe the influence of IL-1β and TNF-α on vimentin expressions in cultured human retinal pigment epithelial (RPE) cells.MethodsExpressions of vimentin exposed to IL-1β and/or TNF-α were detected with immunohischemistry and color imaging system.ResultsIL-1β and/or TNF-α (20 ng/mL) could upregulate expressions of vimentin.The gray degree values of vimentin expressions were 76.9±8.1 in IL-1β+TNF-α-treated group,78.4±8.1 in IL-1β-treated group and 90.2±6.7 in TNF-α -treated group respectively with significant differences (P<0.01) in comparison to control group (110.2±10.7).ConclusionIL-1β and/or TNF-α can stimulate expression of vimentin.
出处
《眼科研究》
CSCD
北大核心
2004年第4期393-394,共2页
Chinese Ophthalmic Research
