广东汉族人纤溶酶原激活剂抑制物-1基因4G/5G多态性与2型糖尿病肾病的相关性

Relationship between plasminogen activator inhibitor-1 gene 4G/5G polymorphism and type 2 diabetic nephropathy in Chinese Han patients in Guangdong Province

目的探讨纤溶酶原激活剂抑制物-1(PAI-1)基因4G/5G多态性与广东汉族人2型糖尿病肾病(DN)发病的关系.方法采用聚合酶链反应-限制性片段长度多态性分析,对147例病史7年以上的2型糖尿病患者[其中DN患者77例,糖尿病非肾病(DNN)患者70例]和26名健康人进行了PAI-1基因4G/5G多态性分析.结果DNN组PAI-1基因的4G/4G基因型、基因频率显著高于DN组(x2=13.008,P＜0.01);与5G/5G基因型相比,DNN组PAI-1基因4G/4G型对DN组的比数比为1.447,95%的可信区间:0.533～3.934(P＜0.05),差异有显著性.而DN与DNN组PAI-1基因4G等位基因频率无显著差异(x2=1.22,P＞0.05);结论PAI-1基因4G/4G基因型与广东汉族人2型DN的发病有关联,4G/4G基因型广东汉族2型糖尿病人易发生DN.

Objective To understand the association between plasminogen activator inhibitor-1 (PAI-1) gene 4G/5G polymorphism and type 2 diabetic nephropathy (DN) in Chinese Han patients in Guangdong Province. Methods PAI-1 gene 4G/5G polymorphism was analyzed in 26 normal individuals, 77 patients with type 2 diabetic nephropathy and 70 type 2 diabetic patients without nephropathy (DNN) by polymerase chain reaction-restriction fragment length polymorphism. Results The 4G/4G genotype frequencies of PAI-1 gene were higher in DN than in DNN patients (0.390 vs 0.171, γ2=13.008, P<0.01), with the odds ratio (OR) of 1.447 (95% confidence interval: 0.533-3.934, P<0.05) in comparison with 5G/5G genotypes between the 2 patient groups. The 4G allele frequencies of PAI-1 gene were similar in DN and DNN groups (0.57 vs 0.51, γ2=1.22, P>0.05). Conclusion The PAI-1 4G/4G genotype is associated with increased risk for type 2 DN the Chinese Han population in Guangdong Province, and type 2 diabetic patients with 4G/4G PAI-1 genotype are more likely to develop DN than those with 5G/5G PAI-1 genotype.