大鼠椎间盘软骨细胞中Bax、Bcl-2及Caspase-8的表达

The studies on expression of Bax、Bcl-2 and Caspase-8 in intervertebral disc chondrocytes of rat

目的:探讨椎间盘软骨细胞在正常与凋亡状态下,Bax、Bcl-2及Caspase-8的表达变化情况。方法:使用酶消化法获取雄性SD大鼠椎间盘软骨细胞,抗Fas抗体诱导凋亡,免疫组化法(SP)检测大鼠正常与凋亡椎间盘软骨细胞的Bax、Bcl-2及Caspase-8的表达,CMIAS-99B型医学图像系统进行表达强度的分析,进而比较分析大鼠正常与凋亡椎间盘软骨细胞的Bax、Bcl-2及Caspase-8的表达变化情况。结果:正常组的Bax和Caspase-8轻度表达,凋亡模型组的Bax和Caspase-8表达明显;而正常组的Bcl-2高度表达,凋亡模型组的Bcl-2轻度表达,正常和凋亡各组均有非常显著差异(P<0.01)。结论:凋亡的大鼠椎间盘软骨细胞内Bax、Bcl-2、Caspase-8的表达与正常状态相比有明显变化。

Objective:To explore the expression of Bcl-2,Bax and Caspase-8 in apoptosis intervertebral disc chonchrocytes of rats induced by anti-Fas antibody.Method:The intervertebral disc endplate of rats were digested enzymatically.The apoptosis of the chondrocytes induced by anti-Fas antibody.The expression of Bcl-2 and Bax,Caspase-8 were determined by immunhistochemical analysis.Result:The expression of Bax and Caspase-8 in the apoptosis cells were higher than those from normal controls(P<0.01).On the contrary,the expression of Bcl-2 of normal cells were higher than those from apoptosis controls(P<0.01).Conclusion:The expression of Bcl-2 and Bax,Caspase-8 of the apoptosis intervertebral disc chonchrocytes of rats were different form the normal controls.