固定化细胞技术降解直链烷基苯磺酸钠的实验研究

STUDY ON THE DEGRADATION OF LINEAR ALKYLBENZENE SULFONATE BY IMMOBILIZED LAS1 STRAIN

目的探讨固定化细胞技术对直链烷基苯磺酸钠 (LAS)降解效果及其影响因素 ,为含LAS废水的处理提供有效方法。方法以海藻酸钠作为从环境中筛选的LAS降解菌LAS1的固定化载体 ,通过摇床恒温培养观察其对LAS的降解效果。结果用固定化细胞技术降解直链烷基苯磺酸钠 ,其降解反应为一级动力学 ,最适降解温度为 30℃ ,并具有较宽的pH值作用范围 ,在pH值 5～ 9范围内其对LAS均有一定的降解效果 ,同时其耐受高浓度LAS的能力较悬浮态LAS1明显增强 ,当LAS浓度达到 110mg·L- 1时仍具有 98%的降解效果 ,在单位时间内降解LAS的能力是悬浮态LAS的 5倍。结论固定化LAS1具有较强的LAS降解能力 ,其所需要的作用条件比较温和 ,具有应用于实际含LAS废水处理的前景。

Objictive To study the effect of immobilized cell technique on the degradation of linear alkylbenzene sulfonate(LAS),so as to provide an effective method for the treatment of waste water containing LAS.Methods Sodium alginate was selected as the carrier for immobilization of LAS1, and the degradation effect of LAS was observed by culturing the immobilized LAS1 at the constant temperature and shaking rate.LAS was determined by a two-phase titrimetric method with amaranth as an indicator. Results The immobilized LAS1 had the capability to degrade LAS.The optimum temperature for immobilized LAS1 was 30℃,and it could degrade LAS in a wide pH range(5～9).The capability of immobilized LAS1 to endure high concentration of LAS was stronger than that of dissociative LAS1.The former still had 98% of degradation rate when the concentration of LAS reached 110 mg.L -1.The degradation activity of immobilized LAS1 was 5 times higher than that of dissociative LAS1. Conclusion The immobilized LAS1 has strong capability to degrade LAS under mild conditions. There is a good prospect of using immobilized LAS1 to treat the waste water containing LAS.