青银注射液中绿原酸与樟脑的含量测定

Determination of chlorogenic acid and camphor in Qingyin Injection

目的 :建立青银注射液 (青蒿 ,金银花 )质量标准中的含量测定项。方法 :用高效液相色谱法和气相色谱法 ,分别测定青银注射液中绿原酸与樟脑的含量。结果 :绿原酸对照品进样量在 0 .0 4 76～ 0 .380 8μg范围内呈良好的线性关系 (r =0 .9999) ,平均回收率为 96 .14 % ,RSD为 0 .88% (n =6 ) ;樟脑对照品进样量在 0 .4 6 2～ 2 .77μg之间线性关系良好 (r =0 .9999) ,平均回收率为 10 0 .2 2 % ,RSD为 2 .4 4 % (n =6 )。结论 :方法简便快速 ,准确可靠 。

AIM: To establish the determination of quality standard for Qingyin Injection(Herba Artemisiae Annuae, Flos Lonicerae). METHODS: The contents of chlorogenic acid and camphor in Qingyin Injection were determined by the HPLC and GC, respectively. RESULTS: Chlorogenic acid showed good linear relationship in the range of 0.0476～0.3808μg(r=0.9999). The average recovery was 96.14%,RSD=0.88%(n=6); Camphor showed a good linear relationship at the range of 0.462～2.77μg(r=0.9999). The average recovery was 100.22%, RSD=2.44%(n=6), respectively. CONCLUSION: The method is simple, quick, accurate and reliable, and can be used for the determination of this preparation.