毛细管气相色谱法测定八味檀香丸中丁香酚的含量

Determination of eugenol in Bawei Tanxiang Pills by Capillary Gas Chromatography

目的 :建立CGC法测定八味檀香丸 (檀香、丁香等 )中丁香酚的含量。方法 :以环己酮为内标物 ,色谱柱为SE 5 4石英毛细管柱 ,用程序升温气相色谱法。结果 :丁香酚进样在 0 .2 5～ 8μg范围内 ,丁香酚浓度与丁香酚峰和内标物峰面积之比值呈良好的线性关系 ,r =0 .9993,平均回收率为 96 .5 % ,RSD为 2 .4 %。结论 :本法操作简便 ,结果准确 ,重现性好 。

AIM: To establish a capillary GC-FID programmed temperature method for determining eugenol in Bawei Tanxiang Pills(Lignum Santali Albi, Flos Caryophylii, etc.). METHODS: Chromatography was performed on a 25m×0.25mm(0.25μm film thickness), SE-54 fused-silica capillary column coupled with a FID-detector. The internal standard was cyclohexanone. RESULTS: A good linearity was obtained in the range of 0.25μg～8μg of Eugenol with a correlation coefficient of 0.9993. The average recovery was 96.5%, the RSD was 2.4%. CONCLUSION: The established method was provided to be a reliable quantitative method for quality control of Bawei Tanxiang Pills.