HPLC测定黄连上清片中盐酸小檗碱的含量

Determination of berberine hydrochloride in Huanglianshangqing Tablets by HPLC

目的 :建立测定黄连上清片 (黄连、大黄、连翘等 )中盐酸小檗碱含量的高效液相色谱法。方法 :以DiamonsilTM C18(2 0cm× 4 .6mm ,5 μm)为色谱柱 ,流动相为 0 .0 33mol·L-1磷酸二氢钾溶液 乙腈 (70∶30 ) ,流速为 1.0mL·min-1,检测波长为 2 6 5nm。结果 :盐酸小檗碱线性范围为 0 .2 376～ 0 .5 5 4 4 μg ,r =0 .9999;平均回收率为 98.7% ,RSD为 0 .7% (n =5 )。结论 :用该法测定黄连上清片中盐酸小檗碱的含量 ,操作简单、重复性好。

AIM: To establish HPLC method for the determination of berberine hydrochloride in Huanglianshangqing Tablets(Rhizoma Coptidis, Radix et Rhizoma Rhei, Fructus Forsythiae, etc.). METHODS: The Diamonsil TM C 18(20cm×4.6mm,5μm) was used with 0.033mol·L -1 Potassium dihydrogen phosphate solution-acetonitrile (70∶30) as a mobile phase. The flow rate was 1.0 mL·min -1. The detecting wavelength was 265nm. RESULTS: The linear of berberine hydrochloride was in the range of 0.2376～ 0.5544μg, r= 0.9999. The average recovery was 98.7% and RSD was 0.7%(n=5), respectively. CONCLUSION: The method is simple,rapid and accurate.