多酚类化合物对MTT法测定细胞活性的影响

Influence of polyphenols on MTT method in detecting cell activity

目的 探讨多酚类化合物对MTT法测定细胞活性的影响。方法 测定咖啡酸、咖啡酸苯酯、槲皮素、芦丁、橙皮苷和香叶木素等几种多酚类化合物在不同浓度条件下 ,对MTT法测定肝细胞活性的影响 ,并与3 H TdR测定结果进行比较。结果 咖啡酸、咖啡酸苯酯、槲皮素、芦丁、橙皮苷剂量依赖地使MTT法测定细胞活性的结果偏高 ,结果与3 H TdR不一致。结论 在研究多酚类化合物或含有多酚类化合物的中药对细胞活性影响时 ,需考虑其对MTT测定方法的影响 ,选用3 H

OBJECTIVE To investigate the influeuce of polyphenols on MTT method in detecting the cell activities. METHOD Hepatic cell activities were detected by MTT at different doses of caffeic acid, caffeic acid phenethyl ester, quercetin, rutin, naringenin, diomin, and were compared with the results detected by 3H-TdR.. RESULTS Hepatic cell activities detected by MTT were dose-dependently increased by polyphenols such as caffeic acid, caffeic acid phenethyl ester, quercetin, rutin, naringenin, and were not consistent with that detected by 3H-TdR. CONCLUSION The influeuce of polyphenols on MTT methods must be considered When investigating roles of polyphenols or traditional Chinese medcine with polyphenols in cell activities .