补充烟酰胺对束缚应激大鼠旷场行为的影响

EFFECTS OF NICOTINAMIDE SUPPLEMENT ON BEHAVIORS IN OPEN-FIELD TEST OF RESTRAINED RATS

目的:研究不同烟酸营养状况对应激大鼠行为的影响。方法:100只雄性Wistar大鼠按体重分为10组:对照组、对喂组、缺乏组、低剂量烟酰胺(NAM)补充组和高剂量NAM补充组及此5组对应的21 d束缚应激组。测定大鼠24h尿N1-甲基烟酰胺(NMN)排泄量、旷场行为、血浆皮质醇、NO和肝脏NOS含量。结果:应激大鼠24h尿N1-甲基NMN排泄量减少。烟酸缺乏和应激都可阻碍大鼠体重增加。对照组和低剂量NAM补充组大鼠在旷场实验中的潜伏期相对较短。应激大鼠血浆皮质醇和NO含量比对照组高;高剂量NAM补充组大鼠的血浆皮质醇含量较低。补充NAM大鼠肝脏NOS含量较高。结论:束缚应激可增加大鼠烟酸消耗;适量补充NAM有助于提高大鼠的应激适应性。

Objective: To study the effects of different niacin nutritures on behaviors of stressed rats. Methods: 100 Wistar male rats were assigned into 10 groups:control, pair-fed, nicotinamide (NAM) deficiency (ND), low-dose NAM supplement (LNS 40 mg/kg diet) and high-dose NAM supplement (HNS 500 mg/kg diet), and the corresponding restrained groups. 24 h urinary excretion of N1-methylnicotinamide (NMN), body weight changes, behaviors in the open-field test, plasma cortisol, nitric oxide(NO) and liver nitric oxide synthase(NOS) were determined. Results: Compared with the control group, restrained rats had relatively lower urinary NMN. Both ND and restrainted stress retarded the body weight gains . Rats in the control and LNS groups had relatively shorter latency time in the open-field test. Restrained rats had higher plasma cortisol and NO than those in the corresponding control group. However, rats with HNS had the lowest plasma cortisol concentrations. Dietary supplement of NAM increased liver NOS . Conclusions: Restraint stress may increase niacin depletion and appropriate NAM supplement has positive effects on stress adaptation in rats.