FasL基因转染NIH3T3细胞诱导BT325胶质瘤细胞凋亡作用的研究

Induction of apoptosis of BT325 human glioblastoma by FasL gene transferred NIH3T3 fibroblast

目的探讨FasL基因诱导胶质瘤细胞凋亡的作用。方法以脂质体将FasL基因转染NIH3T3纤维母细胞,采用免疫组化染色法和RT-PCR法检测NIH3T3/FasL细胞中目的基因的表达和转录,Hochest33342荧光染色法和TUNEL法检测共培养条件下观察其对胶质瘤细胞BT325诱导凋亡的作用。结果NIH3T3/FasL细胞能有效表达FasL,与NIH3T3细胞(对照组)相比,有显著诱导BT325细胞凋亡的作用(P﹤0.05)。结论转染了FasL基因的NIH3T3细胞可诱导胶质瘤细胞凋亡。

Objective To study the effect of FasL gene on the induction of apoptosis of human glioma cells in vitro. Methods FasL gene was introduced into NIH3T3 fibroblast cell by liposome reagents. FasL expressed by the NIH3T3/FasL cells was investigated by immunohistochemical staining and FasL mRNA was explored by RT-PCR. The ability of NIH3T3/FasL cells to induce apoptosis of BT325 glioblastoma co-cultured was assayed by Hochest 33342 fluorescent staining and TUNEL method respectively. Results FasL was expressed effectively by NIH3T3/FasL cells. Comparing with the control group, the experimental group had significant effect on the induction of apoptosis of BT325 cells (P<0.05). Conclusion NIH3T3 fibroblast transferred FasL gene can induce apoptosis of glioma.