人精液中一氧化氮和γ-谷氨酰基移换酶活性的关系

NO level and γ-glutamyltransferase activity of human sperm

目的 :探讨人精液中一氧化氮 (NO)与γ 谷氨酰基移换酶 (γ glutamyltransferase,GGT)活性的关系 .方法 :参照WHO标准 ,进行精液常规分析 ,按精子密度 (× 1 0 9/L)、活动率 (% )不同分为 (正常 ,<2 0 ,<2 0～ 4 0 ,>4 0 ) 4个组 .采用镀铜镉还原荧光法检测NO代谢产物硝酸盐 (NO-3 ) .用以Glucana为底物动力学方法测定精液GGT活性 .结果 :89例不育者NO含量为 (78.5± 1 .5 ) μmol/L ,与精子密度 (33.3±1 4 .3)× 1 0 9/L ,活动率 (33.3± 1 4 .3) %及GGT活性 (2 0 3.1 9±35 .89)× 1 0 3 nkat/L呈显著的负相关 (r分别为 - 0 .5 6 ,- 0 .6 3,- 0 .71 ,P <0 .0 1 ) .结论 :精液GGT活性测定是评价附睾头部功能的重要指标 ,提示NO对精液GGT活性有抑制 。

AIM: To study the relationship between the nitric oxide (NO) and the γ glutamyltransferase (GGT) activity of human sperm. METHODS: Sperm routine analysis was conducted following WHO standard. Eighty nine subjects were devided into 4 groups according to the density and activity of sperm. A coppered reduced cadmium fluometric assay was used to detect nitrate, a metablic product of nitric oxide in sperm. The activity of sperm GGT was detected by Glucana method. RESULTS: The content of NO had a significantly negative relationship with the density, the motility rate, and GGT activity of sperms in the 89 infertile patients ( r = -0.56, -0.63, -0.71, P <0.01). CONCLUSION: The detection of the activity of sperm GGT is an effective index to assess the capability of sperms being fertilized. NO inhibits the activity of sperm GGT and affects the maturity and motility of sperms.