膜片钳技术在角膜研究中的应用进展

Patch clamp technique in cornea research

膜片钳技术产生于 1 976年 ,自 2 0世纪 90年代以来 ,这一技术被应用于角膜各层细胞的研究中 .研究结果显示 ,在角膜上皮细胞、内皮细胞上存在着多种离子通道 ,它们对于维持角膜的透明性具有重要意义 .在研究中 ,多种模式的膜片钳技术被应用其中 .研究者不仅记录了角膜多种细胞的离子通道活动 ,还对细胞的静息膜电位和膜电容进行了测量 。

The patch clamp technique was invented in 1976 and has been applied in researches on cornea cells since the 90s of twenty century. It has been found that there are different types of ionic channels on the corneal epithelium and endothelium. These channels are responsible for maintaining cornea transparency. Several patch clamp modes have been used. The activities of ionic channels, the resting potential and capacity have been measured and the expression modulation of the channels has also been preliminarily studied.