胃蛋白酶C基因在大鼠胃底腺发育过程中上皮细胞内的表达

Pepsinogen C Gene Expression in Epithelial Cells of Rat Stomach during Development

目的研究胃蛋白酶原基因C在大鼠胃底腺发育过程中上皮细胞的表达。方法采用地高辛标记的RNA探针,用原位杂交的方法研究了大鼠胃底腺中胃蛋白酶C产生细胞的个体发育。结果大鼠胃腺在胚胎18.5天开始出现,但没有形态分化,胃蛋白酶的mRNA在出生后3.5天首次被原位杂交法检出,其蛋白酶产生细胞在出生后8周发育成熟,胃蛋白酶的mRNA表达在主细胞和颈黏液细胞内,其发育可分为4个阶段①胚胎18.5天至出生后0.5天;②出生后3.5天至2周;③出生后3周至4周;④出生后8周。在胃底腺发育过程中,胃蛋白酶mRNA的表达至局限于某种特定的细胞,这些细胞的分布具有明确的阶段特异性。结论认为在发育生物学的研究中,胃蛋白酶C可作为胃上皮细胞分化的分子标志。

Objective: To study the espression of pepsinogen C gene in epithelial cells in developing rat stomachs. Methods: In this study, the ontogeney of pepsinogen producing cells in rat fundic glands was studied by in situ hybridization vsing a digoxigenin-labeled RNA probe. Results: The rat gastric epithelium was stratified but was morphologically undifferentiated at 18.5 days gestation. The pepsinogen mRNA was expressed both in chief cells and mucous neck cells in adult rats, in which first detected by in situ hybridization in the stomach of the rats at 3.5 days after birth. The development of pepsinogen producing cells could be classified into for stages:①18.5 days gestation to 0.5 days after birth;②3.5 days to 2 weeks atrer birth;③3-4 weeks after birth; ④8 weeks after birth. Pepsinogen expression is strictly limited to these cells and shown a developmetal stage-specific manner. Conclusion: We concluded the pepsinogen C could offer excellent molecular markers of differentiation of stomach epithelial cells in developmental biology.