莪术油对大鼠球囊损伤血管的保护作用及外膜TGF-β1 mRNA表达的影响

Effects of Oleum Curcumae Aromaticae Oil on Injuried Rat Arteries and TGF-β1 mRNA in Adventitial Cells after Balloon Angioplasty

目的研究莪术油注射液对小鼠球囊损伤血管新生内膜形成及血管重构的作用以及外膜TGF鄄β1(转化生长因子鄄β1)mRNA的变化。方法用球囊导管损伤大鼠左颈总动脉制成再狭窄模型,术后7天采用原位杂交技术结合图像分析观测各组外膜TGF鄄β1mRNA的表述情况,术后14天计算机图像分析系统分析形态学及组织学的变化。结果术后7天,假手术组血管外膜细胞极少表达TGF鄄β1mRNA;模型组血管外膜较多的细胞表达TGF鄄β1mRNA,莪术油组阳性表达细胞较模型组明显减少。术后14天,模型组腔面积、外弹力板周长、内弹力板周长较假手术组均明显减少(P<0.01),莪术油组和模型组比较,最大内膜厚度及新生内膜面积明显减少,腔面积、外弹力板周长、内弹力板周长明显增加(P<0郾01),与假手术组比较,无显著性差异(P>0郾05)。结论外膜TGF鄄β1mRNA基因表达的增加是再狭窄的重要机制,莪术油注射液显著抑制了球囊损伤大鼠颈动脉内膜增生和血管重构,作用机制可能与抑制外膜TGF鄄β1mRNA基因表达有关。

Objective: To investigate the changes of TGF-β1 mRNA in adventitial cells and the effects of Oleum Curcumae Aromaticae oil injection on injuried rat arteriesafter balloon angioplasty. Methods: The left common carottid artery in rats was denuded with a balloon catheter. Cross-sections from the injuried segments of the injuried rat arteries at 7 days were processed for the expression of TGF-β1 mRNA in adventitial cells study by in situ hybridization technology and computer-assisted picture andlysis system. Histological and morphological study were processed by computer-assisted picture analysis system at 14 days. Results: Seven days after balloon injury, Arteries of sham-opration group displayed low-level expression of TGF-β1 mRNA in Adventitial cells. Compared with sham-opration group,adventitial cells of model group showed dramatic upregulation of TGF-β1 mRNA that the positive is perinuclear buffy reaction.Oleum Curcumae Aromaticae oil injection caused a marked reduction of expression of TGF-β1 mRNA in adventitial cells.14 days after balloon injury, Lumen area, EEL perimeter, IEL perimeter of model group were decreased significantly(P < 0.01) than those of sham-operation group. Compared with model group, maximal intimal thickness, neointima area of Oleum Curcumae Aromaticae oil group were decreased significantly(P < 0.01), but with no differences compared with those of the sham-operation group(P > 0.05). Lumen area, EEL perimeter, IEL perimeter increased markedly(P < 0.01) up to that of the sham-operation group(P >.05). Conclusion: The expression of TGF-β1 mRNA in adventitial cells is a major cause of restenosis after arterial injury. We concluded that Oleum Curcumae Aromaticae oil injection inhibits neointimal formation and vascular remodeling after arterial injury by inhibiting expression of TGF-β1 mRNA in adventitial eclls.