JWA蛋白与α-微管蛋白结合和对微管稳定性的调节

JWA protein binds to α-tubulin and regulates microtubule stability

目的探讨了JWA蛋白与琢鄄微管蛋白在细胞内的分布和相互关系,特别是在有丝分裂过程中的动态变化及与微管蛋白的关系。方法用荧光显微镜技术研究低温处理、处理后恢复的NIH3T3细胞JWA蛋白和微管蛋白的相互作用;用激光共聚焦技术检测NIH3T3细胞的JWA蛋白与琢鄄微管蛋白在细胞内的分布。结果JWA蛋白在细胞内与琢鄄微管蛋白的分布基本平行。结论JWA蛋白,作为一种新的微管相关蛋白,在微管动力学变化过程和有丝分裂过程中与琢鄄微管蛋白有交互作用,与琢鄄微管蛋白结合,可能对微管稳定性起一定的调节作用。

Objective: JWA gene is a cytoskeleton related gene and associated with cell differentiation and apoptosis. Our recent studies have demonstrated that JWA protein is not only structurally binding to β-tubulin, but a novel functional MAP-contributions to cell shape and cellular amino acid transportation. Methods: To understand if JWA protein is localized to α-tubulin, in this study, we use immunofluorescence microscopy assay for NIH3T3 cell culture models and the results have provided strong evidence for a linkage between JWA protein and α-tubulin. Results: Briefly, JWA protein is stably binding to α-tubulin whenever α-tubulin is temperature-dependently polymerized or not, and it might be critical to the mitosis of the cell. The confocal laser scanning microscopy revealed that the intracellular distribution of JWA protein is obviously different to that of α-tubulin during mitosis. Conclusion: These results suggest that JWA protein binds to α-tubulin, and might be a novel modulator of microtubule.