摘要
以分泌抗人IgG单克隆抗体的杂交瘤细胞为对象 ,通过免疫试验测定了囊素对其第二信使物质的影响。结果表明 ,囊素处理对杂交瘤细胞内cAMP浓度的影响不明显 (P >0 .0 5 ) ,而对cGMP浓度的影响与囊素作用的时间和剂量有关。分别用 5 0 μg mL和 5 0 0 μg mL囊素处理 5min后 ,两组细胞cGMP浓度均显著下降(P <0 .0 5 ) ,导致cAMP cGMP值明显上升 (P <0 .0 5 ) ,10min后 ,前者的cGMP浓度不再变化 ,而后者的cGMP浓度继续下降。提示囊素可能以cGMP浓度或cAMP cGMP值的变化为介导而起作用。
The effects of bursin in different concentration on cAMP and cGMP level of hybridoma cells secreting monoclonal antibody against human IgG were studied by immunoassay. The results showed that the concentration and action period of bursin could not significantly influence the level of cAMP in hybridoma cells (P>0.05), but could influence the level of cGMP. After the cells were treated for 5 minutes with 50μg/mL and 500μg/mL bursin respectively, the level of cGMP in both group of the cells all decreased significantly (P<0.05), which led the ratio of cAMP to cGMP to increase greatly (P<0.05). 10 minutes later, the cGMP level of the latter still decreased, but that of the former remaintained the same. These results indicated that bursin could act through the change of the concentration of cGMP or the value of cAMP/cGMP.
出处
《上海农业学报》
CSCD
2004年第3期114-116,共3页
Acta Agriculturae Shanghai
基金
国家自然科学基金项目 ( 3 0 170 70 2 )
