日本血吸虫线粒体DNA的遗传变异

Studies on the Genetic Variation of Mitochondrial DNA of Chinese Schistosoma japonicum

目的对中国不同自然隔离群的日本血吸虫线粒体DNA进行遗传变异的研究,为中国日本血吸虫地域株的划分提供依据。方法采用聚合酶链反应(PCR)技术对中国7省11地日本血吸虫的线粒体NADH脱氢酶1(ND1)和细胞色素C氧化酶1(CO1)基因片段进行扩增,将扩增产物测序,计算序列遗传距离(D)。用SAS统计分析软件绘制聚类图。结果两片段PCR扩增产物分子量分别为450与470左右;其中中国大陆与中国台湾日本血吸虫之间的D为0.1710～0.5003;中国大陆各地域株之间D为0～0.4179;中国大陆山区型日本血吸虫之间D为0;中国大陆湖沼洲滩型日本血吸虫株之间D为0.0451～0.1481;湖北省境内5个不同地域株之间D为0～0.0112。结论中国日本血吸虫在线粒体DNA水平上至少可分为不同层次的5类:①中国台湾;②云南洱源、四川天全;③湖北5地;④安徽贵池、湖南岳阳、江西新建。其中①与其它余几类在总体上可分为两大类。

Objective To analyze the genetic variation of mitochondrial DNA of different Schistosoma juponicum isolates. Methods Mitochondrial NADH dehydrogenase1(ND1) and cytochrome C oxidase1(CO1) gene fragments were PCR amplified and sequenced. Based on the sequences, the genetic distances were measured. Results The PCR products of the ND1 and CO1 fragments were 450bp and 470bp respectively. The genetic distances between Taiwan and mainland isolates of S.juponicum were 0.1710～0.5003;0～0.4179 in mainland isolates;0 in hilly regions;0.0451～0.1481 in lake regions;0～0.0112 in different regions of Hubei province. Conclusions We suggest that strains of Chinese S. juponicum can be divided into five groups at least in genetic level: ①Taiwan isolate;②Yunnan Eryuan and Sichuan Tianquan;③The five regions of Hubei province; ④Anhui Guichi,Sichuan Tianquan and Jiangxi Xinjian; ⑤Basically, all the isolates can be divided into two big groups, the Taiwan isolates and all the other remaining isolates.