小克银汉霉原生质体的制备与再生

Protoplast formation and regeneration of γ-linlenic acid producing starins of C.echinulata

目的 为了利用微生物发酵生产γ 亚麻酸,研究了小克银汉霉原生质体制备与再生的条件。方法 利用液体振荡培养法,对酶解系统、渗透压稳定剂、菌丝培养时间、酶解温度等因素对小克银汉霉原生质体制备与再生的影响等进行了实验。结果 原生质体的产量可达到5.3×106个/mL,原生质体的再生率达到1.85%。结论 ①用2%的蜗牛酶酶解28℃培养24h的菌丝,以0.7mol/L甘露醇作为渗透压稳定剂,酶解前以0 3%β 巯基乙醇处理30min,便可制备大量的小克银汉原生质体;②用含0.8mol/LKCl的高渗双层培养基包埋制备的原生质体,可得到较高的原生质体再生率。

Aim High yield of protoplasts from C. echinulata was obtained by using a single of 20 mg/mL sailase.Methods The effects of factors,such as mycelial ages,osmoyic stabilizers,enzyme systemand temperature on the formation and regeneration of protoplast in R.arrhizus were systematically studied. Results Optimum conditions for preparation of protoplasts of the fungus were obtained.Conclusion The regeneration was tested on solid media containing osmotic stabilizer.