中药免疫增强剂用于提高吡喹酮杀血吸虫效果的实验研究(英文)

Experimental study on improving schistosomicidal effect of praziquantel by Chinese herbal immunoenhancing reagents

目的观察两种中药免疫增强剂对吡喹酮杀血吸虫效果的影响。方法将昆明小鼠感染日本血吸虫后,第28天开始用两不同中药制剂(即中药1号和中药2号)分别灌胃C组和D组小鼠,0.5ml/只/d,连续1周。感染后第35天给予吡喹酮半剂量进行治疗。同时设单用吡喹酮半剂量治疗对照组,吡喹酮全剂量治疗对照组和不用药感染对照组。感染后第49天剖杀小鼠灌注收集虫体。计算杀虫率、观察小鼠肝切片中虫卵肉芽肿大小并测定小鼠血清中特异性抗体动态水平。结果两种中药制剂分别将吡喹酮的杀虫率提高了33.97%和41.18%:虫卵肉芽肿大小与对照组比差异无显著性;在给中药前各组小鼠血清中特异性抗体水平无显著差别,给中药后,中药组小鼠血清中特异性抗体水平逐渐升高,直到给吡喹酮治疗前的抗体水平均显著高于对照组。结论本研究选用的两种中药制剂均能在不加重虫卵肉芽肿病变的同时提高吡喹酮杀日本血吸虫效果,其作用机制可能与中药增强剂促进宿主免疫应答,增强血吸虫特异性抗体生成有关。

Objiective: To observe the influence of two Chinese medical herbal immunoenhancing reagents with different components on the schistosomicidal effect of praziquantel. Methods: Mice were infected by percutaneous infection 25 Schistosoma japonicum cercariae. After infection, from 28th day to 35th day the animals were fed with heral immunoenhancing reagents 0.5 ml per mouse per day. Then praziquantel were administered to the mice. All the mice were sacrificed at the 49th day after infection. The worms were calculated, liver egg granulomas were observed and the specific antibodies of different period were detected by ELISA. Results: Compared with the control group , the schistosomicidal effect were increased by 33.97% and 41.18% in the two groups treated with praziquantel plus Chinese medical herb. There was no dramatical change in the size of liver egg granulomas. The level of specific antibody gradually elevated when the two Chinese medical herb compounds were fed. It was significantly higher than that of the control group before praziquantel was administered. Conclusions: The two Chinese herb compounds selected can improve the effect of praziquantel in killing Schistosoma japonicum. However, they do not change the size of liver egg granulomas. These may be related to that the herb can improve the host immune response,especially enhance the specific antibody production.