用cDNA表达阵谱分析原癌基因和抑癌基因在胃癌中的表达

Gene expression analysis of pro-onc and tumor suppressor genes in gastric cancer by complementary DNA microarray

目的:从基因水平了解正常胃和胃癌组织原癌基因和抑癌基因表达的分子机制。方法:从正常胃和胃癌组织中提取、纯化mRNA,逆转录合成cDNA,荧光标记后与H80D型芯片杂交,扫描后筛选出表达差异的原癌和抑癌基因。结果:在195条原癌及抑癌基因中,13条存在表达差异,其中6条(3.1%)表达上调,7条(3.6%)表达下调。结论:应用该方法识别出的原癌和抑癌基因对胃癌的诊断和治疗具有潜在的价值。

Objective: To study the expressions of pro-onc and tumor suppressor genes in gastric cancer by cDNA expression array. Methods: The probes were prepared by labeling gastric cancer tissue mRNA and normal gastric mucosa mRNA with Cy5-dUTP and Cy3-dUTP separately through reverse transcription. The mixed probes were hybridized to the cDNA microarray, and the fluorescent signals were scanned to show the difference between 2 kinds of tissues. Results: The comparative analysis revealed that 13 out of 195 pro-onc and tumor suppressor genes had differential expression (>2 fold or <0.5 fold), among which 6 were up-regulated (3.1%) and 7 down-regulated (3.6%). Conclusion: These studies indicate that the genes identified through this approach have potential value in the diagnosis and therapy of gastric cancer.