IL-8在子宫内膜异位症发病中的作用

Role of interleukine-8 in the pathogenesis of endometriosis

目的:探讨白细胞介素8(IL鄄8)与子宫内膜异位症(内异症)发病的关系。方法:内异症患者36例作为研究组,非内异症患者20例作为对照组。ELISA方法测定内异症患者血清及腹水中IL鄄8的表达情况。Northern杂交比较不同类型异位病灶之间以及内异症患者的在位内膜与非内异症的对照子宫内膜之间IL鄄8mRNA表达强度的差异。MTT法分析IL鄄8、anti鄄IL鄄8对体外培养的子宫内膜基质细胞增殖的影响。结果:内异症组腹水中IL鄄8显著高于对照组(P=0.03熏0.003),并且与疾病的严重程度呈正相关(r分别为0.823,0.934)。重度内异症组血清中IL鄄8显著高于轻度组穴P=0.0012雪。血清中IL鄄8与疾病的严重程度无明显相关(r为0.348,P>0.05);IL鄄8mRNA的表达强度:腹膜红色病灶>在位内膜>卵巢巧克力囊肿>宫骶韧带结节。在分泌期,内异症患者的子宫内膜组织中IL鄄8mRNA的表达强度明显高于非内异症患者的在位内膜;IL鄄8刺激内膜基质细胞增殖具有浓度和时间效应熏该作用可被anti鄄IL鄄8抑制。结论:IL鄄8在内异症发病的多个环节如腹腔微环境异常及刺激异位细胞增殖等方面起到重要作用。

Objective: To determine the expression of IL-8 in endometriosis. Methods: Thirty-six patients with endometriosis (EM) were selected as study group. Twenty patients without endometriosis served as control group. IL-8 concentrations in peritoneal fluid and serum of both groups were detected by enzyme linked immunoabsorbent assay (ELISA). The differences of IL-8 mRNA expression in eutopic endometrium of patients between with and without endometriosis, and the differences among different endometriotic lesions were further studied by Northern Blotting. IL-8 and anti-IL-8 were used to treat cultured endometrial stromal cells(ES). Results: The peritoneal fluid and serum from patients with EM contained significantly greater amounts of IL-8 than those in controls(P=0.03,0.003). A significant correlation between peritoneal fluid IL-8 content and the severity of disease was noted, but there were no evidences of a relationship between concentrations of serum IL-8 and the severity of disease. It was showed that the expression density of IL-8 mRNA in study group was significantly higher than in the control group. Among the different endometriotic lesions, the IL-8 mRNA expression density was: red peritoneal lesion(RPL)>ovarian chocolate cyst(OCC)> uterosacral ligament nodule (ULN). There was a dose-dependent stimulatory effect of IL-8 on survival of cells, and this function can be inhibited by anti-IL-8. Conclusion: IL-8 may play an important role in the pathogenesis of endometriosis by promoting the growth of endometrial cells and change peritoneal circumstance.