^(125)IUdR治疗C6脑胶质瘤的实验研究

Therapeutic Effect of ^(125)IUdR on the Glioma Cell Line C6 in vitro and in vivo

目的 探讨 1 2 5IU d R对脑胶质瘤的治疗作用。方法 体外采用细胞生长曲线测定、MTT法和集落形成试验观察 1 2 5IU d R对 C6脑胶质瘤细胞的作用 ;体内运用 Wistar大鼠 C6脑胶质瘤动物模型进行生存分析 (肿瘤增殖高峰期局部缓慢三次注射药物 ,每天每次 7.4× 10 3k Bq。结果 1 2 5IU d R可显著抑制 C6细胞体外增殖 ,具有时间和浓度依赖性。其中 MTT法显示 15 0 k Bq/ ml 1 2 5IUd R作用 5 d后抑制率高达 93.0 6 %。而对照组 Na1 2 5I和 1 2 7IU d R对 C6细胞体外增殖无明显抑制作用。1 2 5IUd R治疗胶质瘤 C6大鼠 5 d后 ,肿瘤重量低于空白组和对照组(P<0 .0 1) ;生存分析显示 :空白组、1 2 7IUd R对照组和实验组的中位生存时间分别为 9d、12 d和 2 7d(P<0 .0 1)。结论 根据肿瘤细胞增殖特征给药 ,1 2 5IUd R可显著抑制脑胶质瘤细胞增殖 ,延长脑胶质瘤

Objective To investigate the therapeutic effect of 125IUdR on gliomas. Methods By means of growth-curve, clonogenic formation experiment and MTT assay, the inhibitive effect of 125IUdR on the proliferation of C6 cells was studied in vitro. Wistar rats with intracerebral C6 gliomas were used to verify the therapeutic efficacy of 125IUdR in vivo. Results C6 monolayer cells were efficiently inhibited by 125IUdR in a time-and-dose-dependent manner. In the MTT assay, after treament with 150 kBq/ml 125IUdR for 5 days, the inhibition rate reached 93.06%. In murine transplantable tumor, 125IUdR had significant therapeutic effect on rats bearing solid tumor glioma C6. After treatment with 125IUdR for 5 days, the tumor weight of experiment group was lower than that of blank group and control group (P<0.01). The median survival of animals treated with 125IUdR(27 days) was markedly longer than that of control group(9 days) (P<0.01). Na 125I and 127IUdR showed little inhibitive effect on the proliferation of C6 cells in vitro and in vivo. Conclusion 125IUdR can markedly inhibit the growth of Glioma cell line C6. 125IUdR has potential for the treatment of malignant brain tumor.