体外循环对心脏瓣膜置换术患者围术期血浆游离氨基酸谱的影响

Impact of Cardiopulmonary Bypass on Free Amino Acid Spectrum in Plasma for Patients with Prosthetic Valve Replacement During the Perioperative Period

目的 了解体外循环对心脏瓣膜置换术患者围术期血浆游离氨基酸谱的影响。方法 采用柱前衍生化的反相高效液相色谱法测定 2 2例心脏瓣膜置换术患者围手术期血浆中 16种游离氨基酸的含量。结果 与术前比较 ,体外循环术后心脏瓣膜置换术患者血浆中多种游离氨基酸含量降低 ,其中以部分生糖氨基酸 (谷氨酸、丙氨酸、丝氨酸、组氨酸 )和支链氨基酸 (亮氨酸、异亮氨酸、缬氨酸 )最为明显 ,差异有统计学意义 (P<0 .0 5 )。结论 体外循环可导致心脏瓣膜置换术患者血浆中多种游离氨基酸含量降低 。

Objective To investigate the impact of cardiopulmonary bypass(CPB) on free amino acid spectrum in plasma for patients with prosthetic valve replacement. Methods The concentrations of the free amino acid in plasma for 22 patients with prosthetic valve replacement were measured with pre-column derivatization of high-performance liquid chromatography. Results Compared with the preoperative data, the concentrations of some free amino acid in plasma decreased after the operation of cardiopulmonary bypass(CPB), especially some of the glucogenic amino acid and branch chain amino acid decreased significantly(P<0.05). Conclusion The operation of cardiopulmonary bypass(CPB) can decrease the concentrations of some free amino acid in plasma for patients with prosthetic valve replacement. It is essential to give these patients proper nutrition support during perioperative period.