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PDGF-AA对血管平滑肌细胞c-myc、c-myb mRNA及PCNA表达的影响 被引量：7

Effect of PDGF-AA on Expression of c-myc,c-myb mRNA,PCNA in Vascular Smooth Muscle Cells
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摘要 目的明确血小板源生长因子 -AA(PDGF -AA)与自发性高血压大鼠血管平滑肌细胞(SHR -VSMC)增殖的关系。方法采用Westernblot、RT -PCR等方法 ,观察在SHR和Wistar大鼠VSMC中 ,PDGF -AA对SHR/Wistar-VSMC增殖细胞核抗原 (PCNA)、c -myc、c -mybmRNA表达影响的差异性。结果在不同浓度PDGF -AA刺激下 ,SHR -VSMC中PCNA、c -myc、c -mybmRNA表达明显高于Wistar-VSMC(P<0.01) ,且呈明显的剂量依赖性关系(P<0.01)。结论PDGF -AA特异性的刺激SHR -VSMC异常增殖 。 Objective To explore the relationship between proliferation of vascular smooth muscle cells and platelet-derived growth factor-AA(PDGF-AA)in spontanous hypertension rats(SHR).Methods Expression of PCNA protein and c-myc、c-myb mRNAin SHR-VSMC and Wistar-VSMC was observed by Western blot.Results The protein of PCNA and mRNA of c-myc、c-myb induced by PDGF-AA were increased in a dose-dependent manner in SHR,but not in Wistar.Conclusions It is suggested that PDGF-AA plays an important role in SHRs for vascular remodeling.
作者 晋军 祝善俊 黄岚 何作云
机构地区 第三军医大学新桥医院心内科
出处 《中国微循环》 2004年第4期218-220,共3页 Journal of Chinese Microcirculation
关键词 血小板源生长因子-AA 血管平滑肌细胞 c-myc、c-myb MRNA PCNA Platelet-derived growth factor Vascular smooth muscle cell C-myc、c-myb mRNA PCNA
分类号 R735.7 [医药卫生—肿瘤]
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参考文献6

  • 1Heldin CH,Westermark B.Mechanism of action and in vivo role of platelet-derived growth factor[J].Physiol Rev.1999,79(4):1283～1316
  • 2晋军,祝善俊,祝之明,杨永健,丁钢.自发性高血压大鼠中血小板源生长因子-AA及其受体表达与血管平滑肌细胞增殖的关系[J].生理学报,2002,54(2):145-148. 被引量：7
  • 3Inagami T,Kambayashi Y,Ichiki T,et al,Angiotensin Ⅱ receptors AT1 and AT2:new mechanisms of signaling and antagonistic effects of AT1 and AT2[J].Jpn Circ J.1997,61:807～813
  • 4Fischer JW,Davies MG,Wight TN.Versican is the predominant proteoglycan in flow accelerated neointimal hyperplasia of endothelialized baboon vascular grafts[J].Circulation.1998,98(suppl Ⅰ ):Ⅰ-228.Abst-ract
  • 5Nakayama M,Fukuda N,Watanabe Y,et al.Low dose of eicosapentaenoic acid inhibits the exaggerated growth of vascular smooth muscle cells from spontanecously hypertensive rats through suppression of transforming growth factor-beta[J].J Hypertens.1999,17(10):1421～1430
  • 6Sabri A,Levy BI,Poitevin P,et al.Differential roles of AT1 and AT2 receptor subtypes in vascular trophic and phenotypic changes in response to stimulation with angiotensin Ⅱ [J].Arterioscler Thromb Vasc Biol.1997,17:257～264

二级参考文献7

  • 1Heldin CH,Westermark B.Mechanism of action and in vivo role of platelet-derived growth factor.Physiol Rev,1999,79(4):1283～1316.
  • 2Inagami T,Kambayashi Y,Ichiki T,Tsuzuki S,Eguchi S,Yamakawa T.AngiotensinⅡ receptors AT1 and AT2:new mechanisms of signaling and antagonistic effects of AT1 and AT2.Jpn Circ J,1997,61: 807～813.
  • 3Fischer JW,Davies MG,Wight TN.Versican is the predominant proteoglycan in flow accelerated neointimal hyperplasia of endothelialized baboon vascular grafts.Circulation,1998,98 (supplⅠ): Ⅰ-228.Abstract.
  • 4Nakayama M,Fukuda N,Watanabe Y,Soma M,Hu WY,Kishioka H,Satoh C,Kubo A,Kanmatsuse K.Low dose of eicosapentaenoic acid inhibits the exaggerated growth of vascular smooth muscle cells from spontaneously hypertensive rats through suppression of transforming growth factor-beta.J Hypertens,1999,17 (10):1421～1430.
  • 5Sabri A,Levy BI,Poitevin P,Caputo L,Faggin E,Marootte F,Rappaport L,Samuel JL.Differential roles of AT1 and AT2 receptor subtypes in vascular trophic and phenotypic changes in response to stimulation with angiotensinⅡ.Arterioscler Thromb Vasc Biol,1997,17: 257～264.
  • 6Parker SB,Dobrian AD,Wade SS,Prewitt RL.AT(1) receptor inhibition does not reduce arterial wall hypertrophy or PDGF-A expression in renal hypertension.Am J Physiol Heart Circ Physiol,2000,278 (2):H613～H622.
  • 7Dobrian A,Wade SS,Prewitt RL.PDGF-A expression correlates with blood pressure and remodeling in 1K1C hypertensive rat arteries.Am J Physiol,1999,276 (6 Pt 2):H2159～H2167.

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中国微循环
2004年 第4期

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